Ó¢ÎÄÃû³Æ £º T7 RNA Polymerase
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»îÁ¦¶¨Òå £º One unit of enzyme incorporates 1 nanomole of AMP into a polynucleotide fraction(adsorbed on DE-81) in 60 minutes at 37¡æ
ÖÊÁ¿¿ØÖÆ £º Tested for the absence of endo-,exodeoxyribonucleases,ribonucleases and for synthesis of strand-specific RNA
²úÆ·ÃèÊö £º Purified from E.coli strains carrying the plasmids encoding the respective RNA polymerases.All those viral RNA polymerases have a stringent specificity for their own promoters and catalyze the synthesis of RNA from ribonucleoside triphosphates in the presence of a DNA template
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